Journals of Gerontology Series A: Biological Sciences and Medical Sciences Large Type Edition
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chang, J.
Right arrow Articles by Richardson, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chang, J.
Right arrow Articles by Richardson, A.
The Journals of Gerontology Series A: Biological Sciences and Medical Sciences 62:223-234 (2007)
© 2007 The Gerontological Society of America

Effect of Aging and Caloric Restriction on the Mitochondrial Proteome

Jinsook Chang, John E. Cornell, Holly Van Remmen, Kevin Hakala, Walter F. Ward and Arlan Richardson

Departments of 1 Physiology, 2 Cellular and Structural Biology, and3 Medicine and 4 Biochemistry and the 5 Barshop Institute for Longevity and Aging Studies at the University of Texas Health Science Center at San Antonio.
6 Geriatric Research, Education and Clinical Center, South Texas Veterans Health Care System, San Antonio.

Address correspondence to Walter F. Ward, PhD, Department of Physiology, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive-MSC 7756, San Antonio, TX 78229-3900. E-mail: wardw{at}uthscsa.edu

The rat mitochondrial proteome was analyzed using two-dimensional polyacrylamide gel electrophoresis (2-D PAGE), and proteins altered by age or caloric restriction (CR) were identified using mass spectrometry. Of 2061 mitochondrial proteins analyzed in the three tissues, a significant change with age occurred in 25 liver proteins (19 increased, 6 decreased), 3 heart proteins (1 increased, 2 decreased), and 5 skeletal muscle proteins (all increased). CR prevented the age-related change in the level of one liver mitochondrial protein, altered the levels of four proteins (one increased, three decreased) from heart, and one protein (decreased) from skeletal muscle. Identification of the proteins that changed with age or CR revealed that they were varied among the three tissues, that is, not one mitochondrial protein was changed, in common, by age or CR in any tissue studied. Thus, the effect of age on the mitochondrial proteome appears to be tissue-specific, and CR has a minor effect on age-related protein changes.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
All GSA journals The Gerontologist
Journals of Gerontology Series B: Psychological Sciences and Social Sciences
Copyright © 2007 by The Gerontological Society of America.